HBV阳性孕妇DC—SIGN／DC—SIGNR基因多态性与宫内感染关系的研究

Relationship between intrauterine infection and the gene polymorphism of DC-SIGN/DC-SIGNR in the pregnant women of HBV positive

目的分析HBV阳性孕妇DC—SIGN与DC—SIGNR的表达水平及其编码基因的多态性与HBV宫内感染相关性。方法应用PCR技术、琼脂糖凝胶电泳检测HBV阳性孕妇DC—SIGN与DC—SIGNR基因的颈区重复序列分型，然后用卡方检验分析DC—SIGN与DC—SIGNR基因各亚型出现的频率在两组中的差异。结果①29例宫内感染组DC—SIGN基因型全部为7／7型的纯合子基因，54例非宫内感染组中出现2例7／5型的基因，其他52例均为7／7型的基因，两组之间差异无统计学意义（P=0．54）。②29例宫内感染组发现4种DC—SIGNR基因型，分别为7／7型、7／5型、9／7型、6／5型，基因型频率分别为0．3793、0．3448、0．2414、0．0345。54例非宫内感染组发现6种基因型，为7／7型、7／5型、9／5型、9／7型、7／6型、6／5型，基因型频率分别为0．5186、0．1481、0．0926、0．1852、0．0370、O．0185。7／5型基因型在宫内感染组（29例）和非宫内感染组（54例）中的分布的差异有统计学意义（P=0．038），其他基因型两组之间差异无统计学意义（P〉0．05）。结论①DC—SIGN编码基因存在个体基因变异，但变异相对较少，在HBV阳性产妇宫内感染组和非宫内感染组中DC—SIGN基因型分布无明显差异。②DC—SIGNR编码基因存在个体基因变异，变异相对较大，DC—SIGNR基因型“7／5型”在HBV阳性产妇宫内感染组和非宫内感染组中分布差异有统计学意义（P=0．038），可能是影响宫内感染的易感基因。

Objective To investigate the influence of the individual genotype differences of DC- SIGN and DC-SIGNR on the mother-to-neonate intrauterine infection of HBV. Methods The genotypes of the gene DC-SIGN and DC-SIGNR in the pregnant women with HBV positive were detected by PCR and agarose gel electrophoresis. The significant difference of gene diversity of DC-SIGN and DC-SIGNR was analyzed by chi-square test. Results （1）All of 29 cases in intrauterine infection group were 7/7 DC-SIGN genotype. In the non-intrauterine infection group, 7/5 genotype were observed in 2 of 54 cases, and the other 52 cases were 7/7 genotype. The two groups was no significant difference （P = 0.54）.（2）29 cases of intrauterine infection group was observed 4 genotypes of DC-SIGNR such as 7/7, 7/5, 9/7 and 6/5, the genotype frequencies were 0. 3793, 0. 3448, 0. 2414 and 0. 0345 respectively. 54 cases of non-intrauterine infection group was found 6 genotypes such as 7/7, 7/5, 9/5, 9/7, 7/6 and 6/5, genotype frequencies were 0. 5186, 0. 1481, 0. 0926, 0. 1852, 0. 0370 and 0. 0185 respectively. The distribution of 7/5 genotype in the intrauterine infection group （29 cases） and the non-intrauterine infection group （54 cases） was statistically significant （P = 0. 038） , and no significant difference was found in other genotypes between the two groups （P 〉 0. 05）. Conclusion The gene DC-SIGN showed relatively little variation in the pregnant women infected with HBV. On the eountrary, there were multiple genotypes of the gene DC-SIGNR in these women, and the genotype ＂7/5＂ of DC-SIGNR might be one of the susceptibility genes associated withintrauterine infection.