摘要
目的:了解尿毒症患者透析前后补体活化与荷C3d免疫复合物(C3d-IC)水平。方法:双抗体夹心ELISA法。结果:透析后C3d含量及C3d-IC水平较透析前显著下降,而C3浓度则显著升高,在所有33例C3d-IC阳性者中,有29例C3d含量高于正常水平,二者水平变化呈现高度的正相关;而透析前的9例C3d-IC阴性者中,C3d含量亦明显增高。结论:尿毒症患者体内补体的激活除主要由C3d-IC引起外,可能还与血清中存在的非免疫性激活物有关。
Objectives:To investigate the complement activation and level change of C3d bearing immune complex(C3d IC) in patients with uraemia pre and post dialysis. Methods: Double antibodies sandwich enzyme linked immunosorbent assay(ELISA) was used. Results: It was found that,compared with pre dialysis,post dialysis C3d concentrations and C3d IC levels were decreased and C3 increased significantly( P <0.01).In all 33 C3d IC positive cases,there were 29 patients C3d concentrations exceeding the upper limit of normal,the level changes of C3d and C3d IC were positive correlation significantly;While,in C3d IC negative cases pre dialysis,C3d concentrations were also significantly increased. Conclusions:The complement activation was,in addition to,mainly influenced by C3d IC,and could be relevent to sera non immune activation substances.
出处
《医学研究生学报》
CAS
1998年第3期31-34,共4页
Journal of Medical Postgraduates