低密度脂蛋白亚组份的分离及初步应用

Isolation of low density lipoprotein subfractions and its application

目的：探讨低密度脂蛋白（ＬＤＬ）亚组份不同特性在动脉粥样硬化中的作用。方法：采用密度梯度超速离心法分离不同密度ＬＤＬ亚组份；分析冠心病（ＣＨＤ）患者和健康人群ＬＤＬ亚组份分布特征。结果：密度梯度超速离心法直接分离出血清中不同密度的ＬＤＬ亚组份，随着密度逐渐增加，电泳迁移率逐渐增大。ＣＨＤ组总ＬＤＬ１、ＬＤＬ２、ＬＤＬ３、ＬＤＬ４显著增高；ＬＤＬ１～４占总ＬＤＬ比例（％）分别为１４．１±４．１、２３．１±７．５、２８．６±９．２、３４．２±１３．７，而对照组为２１．５±１３．５、２１．８±８．７、２７．４±１０．９、２９．３±１３．６，冠心病患者ＬＤＬ分布向小而重ＬＤＬ亚组份倾移。结论：小而重ＬＤＬ亚组份是冠心病发生的危险因素。

Objectives: To observe low density lipoprotein (LDL) subfractions associations with risk of coronary artery diseases(CHD). Methods: LDL subfractions were isolated directed from plasma by non equilibrium density gradiant centrifugation, and distribution of LDL subfractions in CHD patients were analyzed. Results:The electrophoretic mobility of LDL subfractions was great along the density increasing; the frequency distributions of LDL subfractions in CHD patients were significantly different from that of control. Conclusions: These data support small, density LDL subfraction as a risk factor for atherosclerosis, and this method is useful for investigation of the role of LDL subfractions in development of atherosclerosis.