FISH技术在中国人膀胱尿路上皮癌诊断及复发监测中应用的研究

Diagnosis of Urothelial Carcinoma of Urinary Bladder Using Fluorescence in Situ Hybridization Technique

目的:应用荧光原位杂交(FISH)技术分析中国人膀胱尿路上皮癌中染色体拷贝数异常情况,评估FISH技术在中国人群中膀胱尿路上皮癌诊断及监测复发中的作用。方法:应用CSP3/CSP7、CSP17/GLP16两组混合探针,通过在膀胱癌病理石蜡切片标本上进行FISH反应,统计染色体拷贝异常情况,并分析其与肿瘤病理分期、分级,肿瘤大小、复发的关系以及染色体畸变率组合诊断膀胱尿路上皮癌的阳性率。结果:3、7、17号染色体及9p21区域的畸变率分别为53.2%、59.7%、61%和57.1%,染色体拷贝数异常与肿瘤分期无相关性(P>0.05),3、7、17号染色体畸变与病理分级有显著相关性(P<0.01),3、7、17号染色体及9p21区域畸变与复发显著相关(P<0.01)9p21区域畸变情况与肿瘤大小具有相关性(P<0.05),FISH探针组合诊断膀胱尿路上皮癌的总阳性率为72.7%。结论:FISH技术有助于探索3号、7号、17号及9p21区域畸变与膀胱尿路上皮癌的病理分期、分级、肿瘤大小及复发之间的关系,并可以作为预测膀胱尿路上皮癌术后复发的有用的指标。

Objective：To analyze the usefulness of FISH （fluorescence in situ hybridization） in the diagnosis of urothelial carcinoma of urinary bladder in Chinese. Methods：The FISH probes, directqabeled to the peri-centromeric regions of chromosomes 3,7 and 17 as well as the 9p21 locus, were performed on paraffin sections of 77 urinary bladder cancer cases which were selected at random from 2007 to 2010. Results：The rates of aneuploidy of chromosomes 3.7 and 17 were 53.2%.59.7%.61% and 57.1% for aberration of 9p21 locus in urothelial carcinoma. The aberrations of chromosomes 3,7, and 17 were significantly associated with grade and recurrences（P〈0. 01）, hut not with stage（P〉0.05）. The aberrations of 9p21 locus had significantly correlation with recurrences（P〈0.01）, but not with the stage and grade of the tumor（P〉0.05）. Conclusions： FISH technique may be a valuable method for the diagnosis of the patients with urothelial carcinoma.