摘要
To unravel the pharmacological actions of garlic, a simple thiocompound, zajoene, was purified from the fresh cloves. Three tumor cell lines were subjected to treatment with zajoene at different concentrations in vitro and the morphological changes, DNA content of the cells and chromosome DNA fragmentation were observed by light microscopy, flow cytometry and agarose gel electrophoresis respectively. It was found that zajoene induced cell death by apoptosis in human HL60 promyelocytic leukemia cells, MGc803 gastric mucoid adenocarcinoma cells and Molt4 T lymphocyte leukemia cells. Western blot assay showed that z ajoene inhibited protooncogene bcl2 expression in the three different kinds of tumor cells, suggesting that zajoene may be a potential agent for tumor chemotherapy.
To unravel the pharmacological actions of garlic, a simple thio-compound, z-ajoene, was purified from the fresh cloves. Three tumor cell lines were subjected to treatment with z-ajoene at different concentrationsin vitro and the morphological changes, DNA content of the cells and chromosome DNA fragmentation were observed by light microscopy, flow cytometry and agarose gel electrophoresis respectively. It was found that z-ajoene induced cell death by apoptosis in human HL-60 promyelocytic leukemia cells, MGc-803 gastric mucoid adenocarcinoma cells and Molt4 T lymphocyte leukemia cells. Western blot assay showed that z-ajoene Inhibited proto-oncogene bcl-2 expression in the three different kinds of tumor cells, suggesting that z-ajoene may be a potential agent for tumor chemotherapy.
基金
theNationalNaturalScienceFoundationofChina(GrantNo .394 70 16 0 )
MinistryofPublicHealth(GrantNo .94 1 017)
ChineseAcademyofMedicalSciences (GrantNo .9310 0 8)
