摘要
Vernalization is an essential factor which affects the flowering development in cold_requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization , we examined mRNA populations in differently_treated plumules of winter wheat \%(Triticum aestivum \%L. \%cv\% Yanda 1817) using mRNA differential display. One vernalization_ related cDNA clone \%(VRC), VRC54\%, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern blot and sequence analysis indicated that \%VRC54\% was a novel vernalization_related clone found in higher plant which not only might play an important role in the floral induction in vernalization_requiring plants but also was different from the cold_acclimatized genes.
Vernalization is an essential factor which affects the flowering development in cold-requiring plants. There is a key stage of nucleic acid and protein metabolism in the process of vernalization in winter wheat. To probe into the molecular determinants of vernalization, we examined mRNA populations in differently-treated plumules of winter wheat (Triticum aestivum L. cv Yanda 1817) using mRNA differential display. One vernalizationrelated cDNA clone (VRC), VRC54, was identified and was only expressed at the key stage of 20 d vernalization, rather than at other stages of nonvernalization, 4 d vernalization and devernalization. Northern blot and sequence analysis indicated that VRC54 was a novel vernalization-related clone found in higher plant which not only might play an important role in the floral induction in vernalization-requiring plants but also was different from the cold-acclimatized genes.
基金
theNationalNaturalScienceFoundationofChina (GrantNo .39330 0 10 )
theFoundationofChineseAcademyofSciencestoProf.TanKehui
