摘要
Translation accuracy, translation elongation rate and translation processivity are three important parameters for each elongation cycle. To study the role of ribosomal protein in translation elongation, these parameters were measured simultaneously for the first time in three \%Escherichia coli\% T83 mutants \%rpsL, rplX\% and \%rpmA\%. The results suggested that the mutants of ribosomal protein S12(\%rpsL\%) and ribosomal protein L24(\%rplX\%) decreased the processivity of ribosome while the mutant of ribosomal protein L27\%(rpmA)\% had little effect on the processivity; \%rplX\% mutant and \%rpmA\% mutant had higher accuracy; the elongation rate of rplX mutant was faster than that of the wild type, whereas the elongation rate of rpmA mutant was the same as that of the wild type.
Translation accuracy, translation elongation rate and translation processivity are three important parameters for each elongation cycle. To study the role of ribosomal protein in translation elongation, these parameters were measured simultaneously for the first time in threeEscherichia coli T83 mutantsrpsL, rplX andrpmA. The results suggested that the mutants of ribosomal protein S12(rpsL) and ribosomal protein L24(rplX) decreased the processivity of ribosome while the mutant of ribosomal protein L27(rpmA) had little effect on the processivity;rplX mutant andrpmA mutant had higher accuracy; the elongation rate ofrplX mutant was faster than that of the wild type, whereas the elongation rate ofrpmA mutant was the same as that of the wild type.
基金
supportedbytheNationalNaturalScienceFoundationofChina (GrantNos.395 70 16 2394 80 0 14 )
