肝钳与肝门阻断的肝损害及其防治的实验研究

Experimental Study on Damage of Liver Clamp and Hepatic Hilar Vascular Exclusion and Its Prophylaxis and Treatment

本实验观察不同阻断时刻肝钳与肝门阻断的肝损害以及预防性应用抗氧化剂的缓解作用。结果表明：（１）ＳＧＰＴ、ＴＢ在肝钳组的变化比肝门阻断组表现得更为一过性。而肝门阻断给药组的改变要比非给药组的轻微且恢复得快。（２）肝门阻断时，ＭＤＡ含量显著升高，机体的Ｔ－ＡＯＣ和ＳＯＤ下降。而给药组可明显减轻上述改变。（３）随着肝门阻断时间的延长，肝组织损害也相应加重，抗氧化剂的给予缓解了肝缺血和再灌注造成的组织损害。肝钳组由于健肝血供未受干扰，因此，不同阻断时刻肝组织学形态与对照组相似无甚改变。结论：（１）肝钳较肝门阻断的肝损害轻，且实用安全。（２）肝门阻断预防性使用抗氧化剂，可明显减轻肝缺血再灌注损害。（３）肝门阻断时经门静脉较外周静脉输注抗氧化剂，其保护作用更直接。

This experiment observed the liver damage caused by liver clamp and hepatic hilar vascular exclusion during different time and the remittent action of preventive application of antioxident.The results were as follows:(1)The changes of SGPT and TB(total bilirubin) in liver clamp group were more temporal than that in the group of hepatic hilar vascular exclusion,while the changes in the antioxidant group were more slight and recovered more quickly than that in nonantioxidant group.(2)During hepatic hilar vascular exclusion,MDA raised,FAOC and SOD dropped,the above mentioned changes might be improved markedly in antioxidant group.(3)The antioxidant improved the liver tissue damage caused by ischemia and reperfusion,the pathologic changes in liver clamp group were ligher than that in the group of hepatic hilar vascular exclusion.Conclusion:(1)Hepatic damage in liver clamp group is lighter than that in the group of hepatic hilar vascular exclusion,liver clamp method is a safe and useful technique.(2)Antioxidant may clearly improve hepatic damage caused by ischemia and reperfusion.(3)During hepatic hilar vascular exclusion,the infusion of antioxidant through portal vein is better than through peripheral vein.