银染PCR-SSCP方法检测人9种恶性淋巴瘤细胞系p53抑癌基因的突变

Detection of p53 Tumor Suppressor Gene Mutation in Nine Kinds of Malignant Lymphoma Cell Lines Using PCR-SSCP Technique

用银染PCR-SSCP方法检测人9种恶性淋巴瘤细胞系P53基因第5、6、7外显子的突变。检出SU-DHL-1，SU-DHL-4，SU-DHL-6，SU-DHL-8，SU-DHL-10，8392等6种细胞系中有P53基因的点突变。表明在恶性淋巴瘤细胞系中p53基因的突变发生率较高，是其发生发展的重要原因之一。且非同位素银染PCR-SSCP方法用于检测p53基因点突变，具有灵敏性高，重复性好，无放射性，周期短等优点。

By using the technique of silver-stained polymerase chain reaction-mediated single-strand conformational polymorphism (PCR-SSCP) analysis, p53 gene mutation in six out of nine B cell non-Hodgkinslymphoma cell lines was detected. The high frequency of p53 mutation in NHL B cell lines suggests that p53gene alteration may play an important role in lymphomagenesis and/or disease progression in a subset of Bcell lymphoma. It proves that the silver-stained PCR-SSCP analysis has many advantages, such as high sensitivity, no radioactivity, short-performed period and excellent reproducibility, so it can be easily applied.