摘要
In ord,r to study the relatiouship between the expression or PML and the antiproliferative effect or interferon (IFN), prostate cancer cells, PC-3, DU145 and LNCaP were cultured, andtreated with IFN-α. The cell growth. rates were measured by crystal violet assay, and the expressionof PML was detected by immunofluomcent staining with PML Polyclonal antibody. Our study indicated that the growth rates or PC-3 and DU145 were significantly inhibited by IFN-α, whereas nosuch effect can be found on LNCaP cell line. The immunofluorescent staining showed that both thestaining intensity and the number or the PML nuclear boles were significantly increased in PC-3 andDU145 cells, however, no significant changes in the PML nuclear body were found in the IFs-αtreated LNCaP cells. Our study demeastrated that the antiproliferative,effct of IFN in prostate cancer cells may be directly related to the induced expression of PML in response to its treatment. Ourfinding supports the hypothesis that PML may mediate some antiproliferative erfects of IFN.
In ord,r to study the relatiouship between the expression or PML and the antiproliferative effect or interferon (IFN), prostate cancer cells, PC-3, DU145 and LNCaP were cultured, andtreated with IFN-α. The cell growth. rates were measured by crystal violet assay, and the expressionof PML was detected by immunofluomcent staining with PML Polyclonal antibody. Our study indicated that the growth rates or PC-3 and DU145 were significantly inhibited by IFN-α, whereas nosuch effect can be found on LNCaP cell line. The immunofluorescent staining showed that both thestaining intensity and the number or the PML nuclear boles were significantly increased in PC-3 andDU145 cells, however, no significant changes in the PML nuclear body were found in the IFs-αtreated LNCaP cells. Our study demeastrated that the antiproliferative,effct of IFN in prostate cancer cells may be directly related to the induced expression of PML in response to its treatment. Ourfinding supports the hypothesis that PML may mediate some antiproliferative erfects of IFN.
