摘要
AIM To determine the site of production and uptake of tumor necrotic factor alpha (TNFα), and evaluate the relationship between serum TNFα and plasma endotoxin (ET) in rats with acute hemorrhagic necrotic pancreatitis (AHNP). METHODS Sprague Dawley rats were divided into AHNP group and control group ( n =12). AHNP model was induced by retrograde injection of 5% sodium taurocholate via pancreatic bile duct. The blood samples were obtained through portal vein 2 and 6 hours after the operation. RESULTS The contents of TNFα in portal vein were increased rapidly in the development of AHNP. They were lower in hepatic vein (280 59±20 02) and femoral artery (310 82±7 97) than in portal vein (354 91±25 50) ( P <0 05), and higher in femoral artery than in hepatic vein 6 hours after the operation ( P <0 05). TNFα level in plasma was increased significantly when ET level in portal vein showed no increase. CONCLUSION Pancreas, spleen, liver, intestinal tract and lung are the main organs to produce TNFα, and liver is also an important site for TNFα uptake in the development of AHNP. Plasma endotoxin is not a trigger for TNFα release in rats with AHNP.
