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CLONING AND SEQUENCING OF THE FERREDOXIN GENE OF BLUE-GREEN ALGA ANABAENA SIAMENSIS

CLONING AND SEQUENCING OF THE FERREDOXIN GENE OF BLUE-GREEN ALGA ANABAENA SIAMENSIS
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摘要 The structure gene for ferredoxin, petFI, from Anabaena siamensis has been ampli-fied by polymerase chain reaction(PCR) and cloned into cloning vector pGEM-3zf(+). The nucleotidesequence of petFI has been determined with silver staining sequencing method. There is 96. 8% homologybetween coding region of petFI from A. siamensis and that of petFI from A. sp. 7120. Amino acid se-quences of seven strains of blue-green algae are compared. The structure gene for ferredoxin, petFI, from Anabaena siamensis has been ampli-fied by polymerase chain reaction(PCR) and cloned into cloning vector pGEM-3zf(+). The nucleotidesequence of petFI has been determined with silver staining sequencing method. There is 96. 8% homologybetween coding region of petFI from A. siamensis and that of petFI from A. sp. 7120. Amino acid se-quences of seven strains of blue-green algae are compared.
出处 《Chinese Journal of Oceanology and Limnology》 SCIE CAS CSCD 1998年第S1期12-16,共5页 中国海洋湖沼学报(英文版)
关键词 ANABAENA siamensis FERREDOXIN CLONING NUCLEOTIDE SEQUENCE Anabaena siamensis, ferredoxin, cloning, nucleotide sequence
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