摘要
Zygotes of Pacific oyster (Crassostrea gigas) were treated for triploid induction with caffeine (10 mmol/L, 15mmol/L and 20mmol/L) in combination with thermal shocks (at 40 minute post -fertilization) lasting for 5 and 10 minutes. The highest yield of triploids, 41. 5%, was obtained from the treatment with 20 mmol/L caffeine at 34℃ lasting 10 minutes. The triploid levels were less than 30. 0% in other treatments. Triploid indution was more effective in treatment with 15 - 20 mmol/L caffeine at 34 - 38℃ than with lower concentrations of caffeine at temperatures below 32℃. Our results suggest tha triploid induction with caffeine in combination with thermal shocks is less efficient than some other methods reported previously.
Zygotes of Pacific oyster (Crassostrea gigas) were treated for triploid induction with caffeine (10 mmol/L, 15mmol/L and 20mmol/L) in combination with thermal shocks (at 40 minute post-fertilization) lasting for 5 and 10 minutes. The highest yield of triploids, 41.5%, was obtained from the treatment with 20 mmol/L caffeine at 34°C lasting 10 minutes. The triploid levels were less than 30.0% in other treatments. Triploid induction was more effective in treatment with 15–20 mmol/L caffeine at 34–38 °C than with lower concentrations of caffeine at temperatures below 32 °C. Our results suggest that triploid induction with caffeine in combination with thermal shocks is less efficient than some other methods reported previously.
