摘要
Objective To investigate the cleavage activities of ribozyme RCP in blocking HBV gene expression and replication in eukaryotic cells. Methods: The recombinant plasmid PCR/pSVL which can transiently express ribozyme RCP in eukaryotic cells was constructed and introduced into HepG2215 cell line with the technique of lipofectamine-mediated gene transfer. The vector pSVL transfection group served as the control. HB-sAg and HBsAg from culture medium of the cells were tested with solid-phase radioimmunoassay. Results:The cpm obtained from the medium samples showed that the inhibition rate of ribozyme RCP for HBsAg andHBeAg was 26. 7% and 24. 8% respectively in this transient expression system. Conclusion:Hammerhead ribozyme RCP can inhibit to some extent the expression of HBV gene.
Objective To investigate the cleavage activities of ribozyme RCP in blocking HBV gene expression and replication in eukaryotic cells. Methods: The recombinant plasmid PCR/pSVL which can transiently express ribozyme RCP in eukaryotic cells was constructed and introduced into HepG2215 cell line with the technique of lipofectamine-mediated gene transfer. The vector pSVL transfection group served as the control. HB-sAg and HBsAg from culture medium of the cells were tested with solid-phase radioimmunoassay. Results:The cpm obtained from the medium samples showed that the inhibition rate of ribozyme RCP for HBsAg andHBeAg was 26. 7% and 24. 8% respectively in this transient expression system. Conclusion:Hammerhead ribozyme RCP can inhibit to some extent the expression of HBV gene.
