摘要
In order to study the effects of IL-6 on the development of liver injury the distribution and cell localization of IL-6 mRNA and its protein in the liver was observed in rats after they were inflicted with 20%TBSA full-thickness burns and/or intraperitoneal injection of endotoxin (lipopolysaccharide, LPS). 156 Wistar rats were randomized into combined burn-endotixin group (CBEG), simple burn injury group (SBG),simple endotoxin group (SEG ) and normal control group (NCG). The changes of serum IL-6 level, and theexpression and localization of IL-6 and IL-6 mRNA in the liver were determined quantitatively, with imrnunohistochemistry (IHC) and in situ hybridization (ISH). It was found that serum IL-6 level showed 2 peak inthe first half hour and the 6th to 12th h after injury in CBEG and one peak in the 3rd h in SBG and in the 12thto 24th h in SEG. IL-6 was mainly located in the sinusoid endothelial cells and Kupffer cells and IL-6 mRNAwas distributed mainly in Kupffer cells, polymorphonuclear cells and macrophages. In addition, the expression levels of IL-6 and IL-6 mRNA were in agreement with the pathological changes of liver injury.It is concluded that IL-6 is one of the key cytokines in the pathogenesis of liver damages after combinedinjury of burns and endotoxin.
In order to study the effects of IL-6 on the development of liver injury the distribution and cell localization of IL-6 mRNA and its protein in the liver was observed in rats after they were inflicted with 20%TBSA full-thickness burns and/or intraperitoneal injection of endotoxin (lipopolysaccharide, LPS). 156 Wistar rats were randomized into combined burn-endotixin group (CBEG), simple burn injury group (SBG),simple endotoxin group (SEG ) and normal control group (NCG). The changes of serum IL-6 level, and theexpression and localization of IL-6 and IL-6 mRNA in the liver were determined quantitatively, with imrnunohistochemistry (IHC) and in situ hybridization (ISH). It was found that serum IL-6 level showed 2 peak inthe first half hour and the 6th to 12th h after injury in CBEG and one peak in the 3rd h in SBG and in the 12thto 24th h in SEG. IL-6 was mainly located in the sinusoid endothelial cells and Kupffer cells and IL-6 mRNAwas distributed mainly in Kupffer cells, polymorphonuclear cells and macrophages. In addition, the expression levels of IL-6 and IL-6 mRNA were in agreement with the pathological changes of liver injury.It is concluded that IL-6 is one of the key cytokines in the pathogenesis of liver damages after combinedinjury of burns and endotoxin.
