摘要
ＩＮＴＲＯＤＵＣＴＩＯＮＡｌｐｈａｆｅｔｏｐｒｏｔｅｉｎ（ＡＦＰ）ｉｓａｍａｊｏｒｓｅｒｕｍｐｒｏｔｅｉｎｐｒｅｓｅｎｔｉｎｔｈｅｅａｒｌｙｓｔａｇｅｓｏｆｄｅｖｅｌｏｐｍｅｎｔｉｎｍａｍｍａｌｓａｎｄｏｔｈｅｒｖｅｒｔｅｂｒａｔｅｓ，ｗｈｉｃｈｖ...
AIM To study the expression of Fas and Bcl 2 proteins in BEL 7404 human hepatoma cells in order to analyze the possible relationship between cell growth regulation by alpha fetoprotein(AFP) and Fas/Bcl 2 proteins. METHODS BEL 7404 human hepatoma cells were maintained in RPMI 1640 medium supplemented with 10% new born calf serum. Cells adhered to coverslips were used to detect Fas and Bcl 2 protein expression by the avidin biotin complex (ABC) immunocytochemical assay. RESULTS Immunocytochemical study showed that essentially all the BEL 7404 human hepatoma cells could express Fas and Bcl 2 proteins, although in various amounts. No positive staining for Fas and Bcl 2 proteins was observed when cells were incubated with non relevant sera to establish the specificity. CONCLUSION Fas apoptosis signals and Bcl 2 rescue/survival signals from apoptosis are expressed in BEL 7404 human hepatoma cells. The finding strongly implys that AFP mediated cell apoptosis and growth enhancement are potentially associated with Fas and Bcl 2 proteins present in those cells.
