摘要
ＥｆｅｃｔｓｏｆＬａｒｇｉｎｉｎｅｄｕｒｉｎｇｉｓｃｈｅｍｉａｒｅｐｅｒｆｕｓｉｏｎｉｎｊｕｒｙｉｎｒａｔｏｒｔｈｏｔｏｐｉｃｌｉｖｅｒｔｒａｎｓｐｌａｎｔａｔｉｏｎＤＩＡＯＴｏｎｇＪｉｎ１，ＹＡＯＸｉａｏＰｉｎｇ２，ＪＩＢｉｎｇ１，ＹＡＮＧ...
Abstract AIM To investigate the functional of L arginine in grafted liver and lung during ischemia reperfusion injury (I RI) in rat orthotopic liver transplantation (OLT). METHODS Using a syngeneic Wistar rat orthotopic liver transplantation (OLT) model 66 rats were randomly divided into three groups ( n =11): L arginine (LA), nitric oxide inhibitor (L NAME, LN) and Lactated Ringer's (LR) solution. After 6 hours of preservation, orthotopic implantation of the donor liver was performed. RESULTS Serum nitric oxide and cGMP levels in the grafted liver tissues lowered immediately upon reperfusion in rat orthotopic liver transplantation. L arginine was used to supplement the nitric oxide synthesizing pathway (NOSP), which prolonged of the survival of the recipient with a one week survival of 20% for LR and 80% for LA liver ( n =6, 28 8d±36 6d, P <0 01 vs LR), and improved the hepatic function. Histopathology indicates that L arginine can maintain the integrity of hepatocytes, improve the hepatic regeneration and protect the lung from injury during cold hepatic I RI . In contrast, the grafted liver function and survival was inferior in all cases with inhibited nitric oxide production. Furthermore, histopathology revealed the intrahepatic thrombosis, more severe destruction of the grafted liver and lung tissues, and more severe inflammatory response of the recipient after inhibition of nitric oxide. CONCLUSION L arginine in a storage solution to supplementing NOSP can protect the grafted liver and the lungs from injury during ischemia reperfusion in orthotopic liver transplantation (OLT). The nitric oxide synthesizing pathway (NOSP) in liver transplantation may be a critical determinant of successful organ transplantation, thus providing a useful pharmacological approach to enhancing liver preservation.
