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应用扩增直接重复序列DNA片段对泰国结核分支杆菌菌株基因差异的研究

The genetic diversity of Mycobacterium tuberculosis strains in Thailand studied by amplification of DNA segments containing direct repetitive sequences
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摘要 背景:发展并使用一种用于研究结核分支杆菌基因差异的聚合酶链反应(PCR)设计:结核分支杆菌H_(37)Rv的两个多态性DNA片段被鉴定和测序。然后引物被设计以用于对两个多态性片段同时进行扩增。该方法被用于研究179例临床结核菌分离株,这些菌株以前曾用IS6110的Southern杂交鉴定。结果:两个多态性片段均含有直接重复序列。其中一个片段的直接重复序列位于α-异丙基苹果酸酯合成酶基因的编码序列之间。179个菌株的两个片段经过扩增后,PCR产物的40个型能够被鉴定。该方法能够将38个IS6110单带菌株区分为23个型。属于北京家族的菌株中的大多数具有与H_(37)Rv相同的PCR产物。Nonthaburi组成员的PCR产物彼此间是相似的。结论:这些结果符合北京家族和Nonthaburi组成员起源于两个共同祖先的假说。该PCR方法可能有助于对含IS6110单拷贝的结核菌菌株进行区分。
作者 W.Namwat P.Luangsuk P.Palittapongarnpim 丁北川
机构地区 Department of Microbiology Depertment of Microbioloty Department of Micrbiology
出处 《国际结核病与肺部疾病杂志》 1998年第1期12-18,共7页 The International Journal of Tuberculosis and Lung Disease
关键词 重复序列 结核分支杆菌 泰国 聚合酶链反应 流行病学
分类号 R346 [医药卫生—基础医学]
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参考文献24

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二级参考文献26

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国际结核病与肺部疾病杂志
1998年 第1期

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