急性病毒性肝炎患者中庚型肝炎病毒RNA检测研究

DETECTION OF SERUM HGV RNA IN PATIENTS WITH ACUTE HEPATITIS

为观察庚型肝炎病毒在急性病毒性肝炎中感染情况,对239例急性肝炎患者的血清,采用两步法筛选进行HGV RNA的检测,并经HGV RNA确证试验,结果显示:HGV RNA阳性者9例,其中合并乙肝1例,合并丙肝3例,合并戊肝1例,非甲-戊型肝炎中占4例。提示:①HGV与其它型肝炎病毒重叠感染较多。②6例患者无输血史,而HGV RNA仍为阳性,提示HGV的输血外传播。③NA-E急性肝炎患者中,HGV的阳性率为23.5％,证实HGV为其病原之一,同时提示还可能存在其它的致病因子。

PURPOSE To investigate the prevalence of HGV infection in patients with acute hepatitis.METHODS Sera from 239 patients with acute hepatitis were detected for the presence of HGV RNAby two-step RT-PCR using primers derived from three different regions of HGV genome (5 '-UTR.NS3 and NS5).RESULTS 9 of 239 patients were HGV RNA positive,including 1 coinfected with HBV,3 with HCVand 1 with HEV,and 4 patients with non A-E hepatitis.CONCLUSION (1) There was a high prevalence of HGV infection among blood receivers,so it shouldbe necessary to screen this virus in the blood products.(2)There were 6 patients infected with HGVwho denied blood transfusion histories, suggesting the possible other transmission route.(3)The factthat HGV RNA was found in 23.5% of patients with non-A-E hepatitis demonstrates not all etio-logic role of HGV in those hepatitis patients.