不同临床型肝病患者中庚型肝炎病毒感染的研究

HEPATITIS G VIRUS INFECTION IN PATIENTS WITH DIFFERENT CLINICAL TYPES OF LIVER DISEASES

目的:了解不同临床型肝病患者的庚型肝炎病毒(HGV)感染状况。方法:应用酶联免疫法(ELISA)检测不同临床型肝病患者血清中抗-HGV,并对抗-HGV阳性血清应用逆转录套式聚合酶链反应法(RT-nPCR)检测HGV RNA。结果:肝硬变,慢性乙型和丙型肝炎病人及HBsAg携带者的抗-HGV阳性率(分别为36.36％、26.2％、12.5％和12.0％),均显著高于急性肝炎(4.17％)。急性和慢性非甲-戊型肝炎病人的抗-HGV阳性率也较高,分别为33.3％(1/3)和16.67％(1/6)。各临床型肝病患者中,抗-HGV阳性和阴性组血清天门冬氨酸转氨酶(AST)和丙氨酸转氨酶(ALT)水平无明显差异。结论:HGV与乙型和丙型肝炎病毒(HBV和HCV)具有较高的共同感染率,部分非甲-戊型肝炎为HGV感染;重叠感染HGV似并不加重肝损害程度。

Objective To detect serum hepatitis G virus markers in patients with different clinical types of liver diseases.Methods An enzyme ~ linked immunosorbent assay was used for detection of serum anti-HGV.The anti-HGV positive sera were also tested for HGV RNA by a reverse trascription nested polymerase chain reactin (RT-nPCK).Results The anti-HGV positive rates in patients with cirrhosis, chronic hepatitis H,C and asymptomatic HBsAg carriers(36.36% ,26.20% , 12.5% , 12.0% , respectively)were significantly higher than that in those with acute hepatitis(4.17%).Patients with acute or chronic non-A-E hepatitis also had a higher anti-HGV positive rate( 1 /3 and 1 /6, respectively).No difference was found in AST and ALT levels between anti-HGV positive and negative groups.Conclusion HGV may have coinfection with hepatitis B and C viruses and 16.67% of non-A-E hepatitis patients are likely infected with HGV.Coinfection or superinfection of HGV does not seem to aggravate the liver injury of patients.