满江红鱼腥藻glnA启动子/GUS嵌合质粒在烟草中的表达

The Expression of Anabaena azollae glnA Promoter/GUSChimeric Gene in Transgenic Tobacco

将满江红鱼腥藻ｇｌｎＡ启动区与ＧＵＳ报告基因连接，构成表达载体并导入烟草，在茎、叶中得到表达．这一结果为研究原核蓝藻与高等植物间转录因子识别、基因表达调控提供了有价值的信息，同时为构建实用载体开拓了新路．

 binary vector was constructed by inserting Anabaena azollae  glnA promoter upstream of GUS reporter gene in an orientation. This expression vector was transformed into tobacco genomic DNA via Agrobacterium tumefaciens LBA4404mediated procedure. The transgenic tobaccos were obtained and the GUS activities in tobacco leaves and stems were examined histochemically. The result provided valuable information for the recognition of transcriptional factors the regulation of genes expression between prokaryotic cynobacteria and higher plant. This opens a new way for the construction of practical vectors.