匹罗卡品家兔点眼对葡萄膜巩膜途径房水流出量的影响

EFFECTS OF TOPICAL PILOCARPINE ON UVEOSCLERAL OUTFLOW IN RABBITS

为探讨匹罗卡品家兔点眼对葡萄膜巩膜途径房水流出色的影响，用1％匹罗卡品（Pilocarpine，PC）对家免单侧点眼。并用0.1mmol／L异硫氰酸荧光素牛血清白蛋白（Fluoreceinisothlocyanate－bovineserumalbumin，FITC－BSA）持续前房灌注30min。测定前、后葡萄膜等6种组织的FITC－BSA含量，计算每眼的葡萄膜巩膜层水流出量（Uveosderdoutflow，Fu）。分别比较点眼前后眼压和点眼后双眼的Fu。结果示：PC点眼后1h处理眼眼压为（0．70±0．08）kPa，下降率为25％（P＜0．01），对照眼眼压无显著变化（P＞0．05）；点眼后2h处理眼Fu值为（0．123±0．026）μl／min，对照眼Fu值为0．177±0．032）μl／min，双眼比较差异有显著性（P＜0．05）。说明1％PC单侧点眼可降低正常家兔眼压，PC可阻断家兔葡萄膜巩膜途径房水外流，兔眼的眼压下降主要为前路排出增加所致。

Effects of topical pilocarpine on uveoscleral outnow(Fu) in rabbits are discussed. 1 %pilocarpine(PC) was unilateraly applied in 5 normal rabbits' eyes. Intraocular pressures(IOP) of bilatera1 eyes were measured before and 1 hour after PC instillation. 0. 1mmol/L fluorecin isothiocyanatebovine serum albumin(FITC-BSA) was Perfused for 30 minutes bilateraly 90 minutes after instillation.Each eye was dissected into anterior uvea, posterior uvea, anterior sclera, posterior sclera, retina and fluid. The amount of FITC-BSA in each tissue was assayed. Fu was obtained according to these amounts. IOPs before and 1 hour after PC instilla tion and Fu of bilatera1 eyes were compared, respec tively. Results: IOP of PC-treated eye was (0. 70±0. 08)kPa 1 hour after instillation. It had a 25 percent decline. The control eye had no significant change on IOP. Fu of PC-treated and control eyes 2hours after instillation was (0. 123 ± 0. 026 ) μl /min and (0. 177 ± 0. 032 ) μl/min, respectively. Signifi cant change existed between bilateral eyes (P ＜0. 05). Conclusion: 1 % PC can decreased IOP after unilateraly topical application. PC can prevent Fu in rabbits. Decrease of IOP following topical PC is dominated by increasing outflow from anterior path