大鼠肝热缺血再灌注后血浆内皮素与6-酮-前列腺素F_(la)变化的观察

AN INVESTIGATION OF CHANGES IN PLASMA ET1 AND 6KetoPGF1a AFTER LIVER ISCHEMIA AND ISCHEMIAREPERFUSION INJURY

目的：肝脏缺血再灌注肝损伤的机理尚未清楚。本研究通过观察大鼠肝缺血和再灌注后血浆内皮素（ＥＴ１）、血栓素Ｂ２（ＴＸＢ２）、６－酮－前列腺素Ｆｌａ、ＳＧＰＴ和病理变化，了解ＥＴ１在肝热缺血再灌注肝损伤中的作用。方法：ＳＤ大鼠３２只分为４组：正常对照组，肝缺血组，再灌注后３０ｍｉｎ和４ｈ组。通过用无损伤动脉夹阻断肝蒂造成大鼠肝缺血动物模型。经腹主动脉采血用放射免疫方法检测血浆ＥＴ１、ＴＸＢ２、６－Ｋｅｔｏ－ＰＧｌａ，同时检测ＳＧＰＴ。统计学方法：ｑ检验，回归相关分析和ｔ检验。结果：肝缺血再灌注导致ＥＴ１和ＳＧＰＴ明显增高（Ｐ＜０．０５），并伴随以６－Ｋｅｔｏ－ＰＧｌａ）增高为主的ＴＸＢ２／６－ｋｅｔｏ－ＰＧＩａ比值失调，这种变化在再灌注４ｈ时尤为明显。此时６－Ｋｅｔｏ－ＰＧＦｌａ与ＥＴ１变化呈正相关，相关系数ｒ＝０．７９（Ｐ＜０．０５）。结论：ＥＴ１是参与肝热缺血及再灌注后肝损伤的主要因素之一。

Objective:This study was designed to investigate the changes in plasma endothelin1(ET1),Thromboxane B2(TXB2),6KetoPGFla and SGPT.Methods:The pathologic changes after liver ischemia by blocking inflow of liver at different time after reperfusion in the SD rats were tecermined.Results:The results showed that ET1and SGPT were increased in plasma after liver ischemia and reperfusion (P<005).The ratio of TXB2/6KetoPGFla was disorder due to obvious increasing of 6KetoPGFla.The most significant change of ratio of TXB2/6KetoPGFla was in 4 hours after reperfusion.The correlativity between ET1 and 6KetoPGFla was positive (r=079,P<005).Conclusion:The ET1 and 6KetoPGFla are belonged these factors which cause liver ischemia and reperfusion injury is this experiment.