去势大鼠血清和骨组织中白细胞介素-6与血清E_2含量的变化及其意义

The Dynamic Determination of Both Contents of IL - 6 and E_2 in Serum and Bone and Data of Bony Histomorphometry in Ovariectomized Rats

目的：观察白细胞介素－6（IL－6）和血清E2对绝经后骨质疏松症的影响，并探讨IL－6与E2的关系。方法：行开腹输卵管峡部结扎后切除双侧卵巢建立绝经后骨质疏松大鼠模型，双抗夹心酶联免疫吸附法（ELISA）检测血清和骨组织培养液（RPML1640）中IL－6含量，血清E2用RIA法测定，同步用IBAS计算机全自动图象分析系统对不脱钙组织行形态计量学指标测量。结果：去势后早期（≤4周）即有血中和骨组织中IL－6升高和E2下降（P＜0．05）。随着去势时间延长（4-22周），骨组织中IL－6浓度高于血中浓度（P＜0．05），血中IL－6与E2呈高度负相关（r＝－0．63，P＜0．01），并出现骨小梁体积减小和骨小梁平均厚度减少以及骨吸收表面增大等骨质疏松形态计量学参数变化（P均＜0．01）。去势大鼠血或骨组织中IL—6浓度在术后4—22周之间升高的程度无显著性，而骨组织中IL-6与骨形态计量学各参数的相关程度依次为RS＞MTT＞FS＞TBV。结论：IL－6可能参与骨质疏松症的发生和发展并造成骨组织形态的改变。

Objective Ovedectomized rats which were used for establishing the model of postmenopausol osteoporsis were ed dynamic determination between IL - 6 and E2 in the serum or bone and Data Of bony histomorphrmetry. Methode 40female SD rats were ramdomly divided into 4 groups, eath group divided into experimental group, Ovariectomized(OV) and contrast group, (sham - ov). Measured with IL - 6 level in serum and bony culture was ELISA, and RIA method was adopted to detemine E2 in serum, and bony histomorphometry was measured with while automobile imaging analysis system. Results In the early period (≤4weeks) after amenorrhea. IL - 6 in serum and bone began to increase and E2 to decrease. With the time going by(4 - 22 weeks), the concentration Of IL - 6 in bone was higher than that in blood(p< 0. 05 ), and IL - 6 and E2 appeared in highly negative connection(r = - 0.63, P< 0. 01 ) , there were no significant changes in the concentration of IL -- 6 in blood or bones of postoperated rat (within4 -- 22 weeks). We found the connective degrees between IL -- 6 level of fore and data bony histomorphometry were: RS > MTT > FS > TBV Conclusion In microsurroundings,the bone of the OV rat discharged more IL.- 6, which might be related to the birth and growth of PMOP and lead to the change of the shape of the bone.