大鼠肠缺血后一氧化氮合酶活性和一氧化氮含量的变化及其意义

Changes in the NO Content and the NOS Activity during Rat Intestinal Ischemia

目的：研究一氧化氮合酶（NOS）及一氧化氮（NO）在肠缺血过程中的交化及其意义。方法：建立大白鼠肠系膜上动脉阻断模型，采用荧光法分别测定回肠组织NO含量及NOS活性。结果：肠缺血早期（1／2h）NO含量及NOS活性变化不大，肠缺血后1～2hNO含量及NOS活性均显著增加。结论：肠缺血后NO含量和NOS活性的增高与肠缺血损伤密切相关。

Objective To study the changes in the NO content and NOS activity and research their physiological significance during the rat intestine ischemia. Methods Eestablish arterial occlusive model in the mesentry and determine the NO content and NOS activity in the lieal tissue respectively. Results There were no obvious changes in the content and the activity early in the intestine ischemia, but after 1 -- 2 hour bob the content and the activity increased cistinctly.Conclusion The change in the NO content corresponds to that in the NOS activity during the intestinal ischemia. Their increases may be one of the great important causes of the intestinal ischemia injury.