恩度对肿瘤上清液诱导的人脐静脉内皮细胞增殖、凋亡的影响及其机制的初步研究

Effects and preliminary study on the mechanism of endostar on proliferation and apoptosis of the HUVECs induced by tumor supernatant fluid

目的:探讨恩度对肾癌ACHN细胞系上清液诱导的人脐静脉内皮细胞(HUVECs)增殖和凋亡的影响及其机制。方法:应用MTT法检测恩度对HUVECs增殖的抑制作用;应用Annexin-V/PI双染法流式细胞仪检测恩度对HUVECs凋亡的影响;Western blot检测恩度对Cbl-b,c-Cbl,磷酸化Akt(p-Akt)和磷酸化ERK(p-ERK)蛋白表达的影响。结果:恩度抑制肿瘤上清液诱导的HUVEC细胞增殖;促进HUVECs凋亡;恩度下调HUVEC中Cbl-b、c-Cbl、p-Akt及p-ERK的表达,且上述效应均呈浓度依赖性。结论:恩度对肿瘤上清液诱导的HUVECs的增殖具有抑制作用,对其凋亡具有促进作用。泛素连接酶、p-Akt及p-ERK通路可能是恩度作用的分子机制之一。

Objective：To explore the effects and the mechanism of endostar on proliferation and apoptosis of the human umbilical vein endothelial cells（HUVECs）induced by renal cell carcinoma（ACHN cell lines）supernatant fluid.Methods： To detect the effects of endostar on the proliferation of the HUVECs by MTT;detect the effects of endostar on the apoptosis of the HUVECs by Annexin V-FITC binding assay;detect the effects of endostar on the expression of Cbl-b,c-Cbl,p-Akt and p-ERK by western blot.Results： Endostar inhibited the proliferation of HUVECs induced by renal cell carcinoma supernatant fluid,promoteed the apoptosis of HUVECs reduced the expression of Cbl-b,c-Cbl,p-Akt and p-ERK in HUVECs,and the effects were depended on the concentration of endostar.Conclusion： Endostar inhibits the proliferation,promotes the apoptosis of HUVECs induced by renal cell carcinoma supernatant fluid,and ubiquitin ligases pathway,p-Akt pathway or p-ERK pathway may be the molecular mechanism of endostar.