凋亡抑制蛋白FLIP在喉部病变组织中的表达及意义

Expression and significance of inhibitor apoptosis protein of FLIP in human laryngeal tissue

目的:检测凋亡抑制蛋白FLIP在人喉部常见病变组织中的表达并揭示其在喉癌等病变发生发展中的意义。方法:采用免疫组织化学EliVision法检测38例喉鳞状细胞癌标本,30例声带息肉组织标本,26例喉乳头状瘤标本,15例正常喉黏膜组织标本,喉癌Hep-2细胞系10个细胞爬片样本中FLIP蛋白的表达并进行统计学分析比较。结果:凋亡抑制蛋白FLIP在38例喉癌标本中有32例阳性表达(84.2%),在喉癌Hep-2细胞系10个细胞爬片样本中全部阳性表达(100%),26例喉乳头状瘤中5例阳性表达(19.2%),30例声带息肉标本中仅2例阳性表达(6.7%),而正常喉黏膜组织中均为阴性。凋亡抑制蛋白FLIP在喉癌组织中表达水平与喉部良性肿瘤和良性病变组织中的表达水平有显著差异(P<0.01)。结论:凋亡抑制蛋白FLIP的表达水平与喉部病变组织的良恶性程度密切相关,提示其可能与喉癌的发生相关。

Objective：To detect the expression of inhibitor apoptosis protein of FLIP in human common laryngeal disease tissues and to explore its significance for the genesis and development of laryngeal carcinoma.Methods： The expression of FLIP in 38 cases of laryngeal squamous carcinoma,30 cases of polyp of vocal cord,26 cases of laryngeal papilloma,15 cases of normal laryngeal tissues and 10 cases of cultured human laryngeal squamous epithelial carcinoma Hep-2 cell were detected by immunohistochemistry method.The different expression levels of FLIP in different human laryngeal tissues were compared statistically.Results： The positive expression of FLIP was found in 32 cases of 38 laryngeal squamous carcinoma（84.2%） and in 10 Hep-2 cell was 100%,in 5 of 26 laryngeal papilloma cases（19.2%）,but in the 30 polyp of vocal cord tissue only 2 cases（6.7%） was positive（P0.01）.Conclusion： The expression of FLIP is closely related to the malignant laryngeal tissue.The result suggested that inhibitor apoptosis protein of FLIP may play a role in the pathway of carcinogensis.