HIF-1α与Caspase-3在乳腺癌中的表达及临床病理意义

Expression and significance of HIF-1α and Caspase-3 in breast cancer

目的:检测HIF-1α、Caspase-3在乳腺浸润性癌、导管内癌、导管内乳头状瘤中的表达,分析两者与乳腺癌发生及临床病理因素的关系。方法:免疫组化法检测HIF-1α、Caspase-3在乳腺浸润性导管癌、导管内癌、导管内乳头状瘤组织中的表达。结果:乳腺浸润性导管癌、导管内癌、导管内乳头状瘤中,HIF-1α阳性表达率分别为68.3%(41/60)、46.7%(28/60)、33.3%(20/60),P<0.05,差异有统计学意义,Caspase-3阳性表达率分别为48.3%(29/60)、58.3%(35/60)、62.8%(47/60),P<0.05,差异有统计学意义;两者表达与浸润性导管癌患者年龄、肿瘤大小无相关性(P>0.05)。HIF-1α表达与乳腺癌病理分期、淋巴结有无转移、组织病理学分级有关(P<0.05)。Caspase-3表达与组织病理学分级有关(P<0.05)。HIF-1α、Caspase-3在乳腺浸润性癌中的表达呈负相关(r=-0.271,P<0.05)。结论:HIF-1α、Caspase-3在乳腺癌的发生中起重要作用,联合检测可作为判断乳腺癌恶性程度和预后的指标。

Objective：To study the expression of HIF-1α and Caspase-3 in invasive ductal carcinoma,intraductal carcinoma in situ and intraductal papilloma and to explore their relationship with breast cancer and clinicopathologic factors.Methods： The expression of HIF-1α and Caspase-3 in patients with invasive ductal carcinoma,intraductal carcinoma in situ and intraductal papilloma were examined by immunohistochemical staining.Results： The positive expression rates of HIF-1α in invasive ductal carcinoma,intraductal carcinoma in situ and intraductal papilloma were 68.3%（41/60）,46.7%（28/60） and 33.3%（20/60）,P0.05.The positive expression rates of Caspase-3 were 48.3%（29/60）,58.3%（35/60）,62.8%（47/60）respectively,P0.05.No relation was seen in their rates of expression with age,and tumor size（P0.05）,the expression of HIF-1α was significantly correlated with lymphatic metastasis,surgical-pathologic staging and histological grade（P0.05）,the expression of Caspase-3 was significantly correlated with histological grade（P0.05）.Expression of HIF-1α was negatively correlated with expression of Caspase-3（r=-0.271,P0.05）.Conclusion： HIF-1α and Caspase-3 were closely related to the advancement and prognosis of breast carcinoma,which are valuable molecular markers to be used for monitoring the disease.