泰乐松注射液的毒理学试验研究

STUDY ON THE TOXICOLOGY TESTS OF TYCLOSONE INJECTION

为提供泰乐松注射液的安全性毒理学评价资料，本试验采用改进Karber法测定小鼠LD_(50)；以剂量递增法测定小鼠蓄积性和耐受性；分别以剂量340、130、30mg／kg为3个试验组．设阴性和阳性对照组进行小鼠骨髓微核试验；分别以剂量500、250、120mg／kg为3个剂量组．设阴性和阳性对照组进行小鼠精于畸形试验。结果表明：其LD_(50)为669．80mg／kg，蓄积系数大于5．3，无明显蓄积毒性和耐受性（P＞0．05），小鼠骨髓微核试验和精子畸形试验结果均为阴性。

In order to provide some scientific data for safety evaluation of Tyclosone injection, thispaper determines LD50 by improved karber method, accumulative toxicity by progressive dose in-crease method, bone marrow micronucleus test with 3 experimental groups at dosage of 340, 130and 30 mg/kg respectively and positive and negative control groups, spermatogenic malformativetest with 3 experimental groups at dosage of 500, 250 and 120mg/kg respectively and positive andnegative control groups. It also determines the tolerance. Experimentive animals used in these 5tests are mice. The data are 669. 80mg/kg(LD50) and over 5. 3 (accumulative coefficient). Theother 3 rasults are all negative.