摘要
Response of biosensor prepared with the thermostable bacterial LDH enzyme was analyzed in the presence of mercury and nickel.For electrode preparation,the enzyme was purified and immobilized on a gold sheet coated by PGA-pyrrole polymeric material.The working electrode was tested at increasing concentration of lactate in the presence of two different concentrations of mercury and nickel.Current response of biosensor decreased from 0.32 μA to 0.09 μA and 4.13 μA to 2.63 μA when 25×10-7 mmol/L mercury and 17×10-5 mmol/L nickel were included in the working solution,respectively.Sensitivity of the electrode decreased from 0.010 2 μA/（mmol·L-1） to 0.0043 μA/（mmol·L-1） in the presence of 25×10-7 mmol/L mercury.On the other hand,the presence of nickel did not result in a decrease in electrode sensitivity.The results pointed out that the prepared biosensor is useful to detect mercury in a sample containing both mercury and nickel together.
分析了采用耐热细菌LDH酶制备的生物传感器对汞和镍的响应性。将酶纯化、固定在一个涂有PGA-吡咯聚合材料的金箔上作为工作电极,在增加溶液中乳酸浓度的情况下测定生物传感器对两种不同浓度的汞和镍溶液的响应性。当溶液中含25×10-7mmol/L汞和17×10-5mmol/L镍时,生物传感器的电流响应分别从0.32μA下降到0.09μA和从4.13μA下降到2.63μA。在含25×10-7mmol/L汞溶液中,生物传感器的敏感性从0.0102μA/(mmol·L-1)下降到0.0043μA/(mmol·L-1)。另一方面,镍的存在并没有导致传感器敏感性的降低。结果表明,制备的生物传感器适合用于含汞和镍溶液中的汞检测。
