摘要
The recombinant DNA techniques are used to construct a fusion protein PBVIL-2/PS-9 which contains gene fragments encoding human IL-2 and a murine single chain antibody (scFv) against human adenocarcinoma. The expression differs from previous reports. It has been expressed as cytoplasmic bodies in E. colt. A high level expression at a level of 40% of total bacterlal proteins is obtained. The fusion protein possesses both the antigen binding characteristics of the parental mAB PS-9 and the bioactivity of IL-2.
The recombinant DNA techniques are used to construct a fusion protein pBVIL-2/PS-9 which contains gene fragments encoding human IL-2 and a murine single chain antibody (scFv) against human adenocarcinoma. The expression differs from previous reports. It has been expressed as cytoplasmic bodies in E. coli. A high level expression at a level of 30 - 40% of total bacterial proteins is obtained. The fusion protein possesses both the antigen binding characteristics of the parental mAB PS-9 and the bioactivity of IL-2.
