摘要
The YAC contig construction has been done for the Human X chromosome short armXp21.3—p11.3, a region which contains several genetic disease gene loci and is of highlybiomedical importance. Using known probes(OTC, DXS166, DMDcDNA) and STS markersof this region, YAC screenings are performed by both YAC colony in situ hybridization andPCR methods. Totally 55 YACs are obtained from the YAC libraries of CEPH, ICRF andthe Institute. The size determination, the analysis of 26 pairs of microsatelite STS, the single copy probe hybridization and the Alu-PCR fingerprinting are performed for these YACs.The mapping of these YACs is performed, and finally, 6 YAC contigs in Xp21.3—11.3 are obtained, covering about 15 Mb. This work will greatly facilitate the positional cloning of disease genes or the genome sequencing in this important region.
The YAC contig construction has been done for the Human X chromosome short arm Xp21.3—p11.3, a region which contains several genetic disease gene loci and is of highly biomedical importance. Using known probes(OTC, DXS166, DMDcDNA) and STS markers of this region, YAC screenings are performed by both YAC colony in situ hybridization and PCR methods. Totally 55 YACs are obtained from the YAC libraries of CEPH, ICRF and the Institute. The size determination, the analysis of 26 pairs of microsatelite STS, the single copy probe hybridization and the Alu-PCR fingerprinting are performed for these YACs. The mapping of these YACs is performed, and finally, 6 YAC contigs in Xp21.3—11.3 are obtained, covering about 15 Mb. This work will greatly facilitate the positional cloning of disease genes or the genome sequencing in this important region.
基金
Supported by the High Technology Research and Development Programme of China
the National Natural Science Foundation of China
