摘要
Objective To observe the changes of proto oncogene c fos/c myc expression and its relation to specific transcription factors in human senescent fibroblast after epidermal growth factor (EGF) addition. Methods The c fos/c myc expression were analysed by Northern blot. Transcription factors were analysed by Southwestern blot. Results (1) The expression of c fos/c myc was less susceptible to induction by EGF as the cells aged. (2) EGF increased the binding of protein P91 to c fos with regulatory sequence and P80 to c myc with regulatory sequence, but the increased levels of both proteins were significantly reduced in senescent cells. Conclusions Inability of c fos/c myc gene induction by EGF in senescent cells might be correlated with some DNA binding proteins.
Objective To observe the changes of proto oncogene c fos/c myc expression and its relation to specific transcription factors in human senescent fibroblast after epidermal growth factor (EGF) addition. Methods The c fos/c myc expression were analysed by Northern blot. Transcription factors were analysed by Southwestern blot. Results (1) The expression of c fos/c myc was less susceptible to induction by EGF as the cells aged. (2) EGF increased the binding of protein P91 to c fos with regulatory sequence and P80 to c myc with regulatory sequence, but the increased levels of both proteins were significantly reduced in senescent cells. Conclusions Inability of c fos/c myc gene induction by EGF in senescent cells might be correlated with some DNA binding proteins.
