人胃癌裸鼠体内原位移植生长过程的动态观察

Developmental stages of orthotopic transplanted human stomach cancer in nude mice

目的 :建立与临床胃癌的生长过程和特点相似实验动物模型。 方法 :以 SGC- 790 1人胃癌细胞株裸鼠皮下移植瘤为材料 ,将瘤组织块种植到裸鼠胃壁 ,动态观察生长过程。 结果 :原位移植模型潜伏期 2～ 4周 ,第 4周以后原位肿瘤明显长出 ,第 12周以后出现肝转移 ,第 16周以后部分荷瘤鼠发生腹水 ,幽门梗阻 ,动物逐渐衰竭 ,出现恶病质等征象。 结论 :原位肿瘤模型与临床胃癌的生长过程和生物学特性非常相似 。

Objectives:To develop an animal model with similar course and characteris- tics of human gastric cancer. Methods:Orthotopic model of human stomach cancer was es- tablished in2 4BAL B/C- nu/nu nude mice by transplanted SGC- 790 1 human gastric cancer cell lines subcutaneously into nude mice.The animals were randomly divided into6 groups. Tumor- bearing nude mice were killed periodically(2 ,4,8,1 2 ,1 6 weeks respectively) after the operation,and the rest 4animals underwent autopsy. Results:Orthotopic tumors devel- oped2～ 4weeks after the operation and grew grandually,liver metastases developed1 2 weeks after the operation,some animals suffered from pyloric obstruction and asites.The survival time was1 6～ 2 4weeks. Conclusions:This'patient- like'orthotopic model might be useful in the study of human stomach cancer.