反相高效液相色谱法测定奎尼丁血药浓度

Determination of quinidine levels in human plasma by HPLC

目的 :建立适合临床检测奎尼丁血药浓度的反相高效液相色谱法。 方法 :血样在碱性条件下 ,经甲醇和氯仿沉淀提取 ,流动相重组进样 ,色谱分析条件为 C18反相柱作分析柱 ,流动相组成为甲醇∶乙腈∶水∶正丁胺∶醋酸 =80∶ 2 0∶ 10 0∶ 0 .4∶ 0 .4 ( v/ v) ,紫外检测波长 334 nm。 结果 :最低检测浓度为 0 .599μmol/ L,线性范围 1.36～ 2 7.2 μmol/ L( r=0 .9992 ) ,平均回收率为 83.9% ,日内、日间的相对标准偏差 <5%。 结论 :本法准确、灵敏度高 。

Objectives:To develop a sensitive and specific reversed- phase high perfor- mance liquid chromatographic method for the determination of quinidine levels in human plasma. Methods:The assay involved a simple extraction procedure. The mobile phase consisted of methanol(40 % ) ,acetonitrile(1 0 % ) and distilled water(5 0 % ) ,p H=4.8. Seperation was achieved on a C1 8ODScolumn and the effluent was measured for UV absorp- tion at334nm. Results:The linear range of drug concentration in the plasma was1 .36～ 2 7.2 μmol/ L (r=0 .9992 ) .The recovery from the plasma was over80 % and the detection limit was 0 .5 99μmol/ L .The RSD% of the assay for both within day and between day was less than5 % .Samples from6 patients had been determined. Conclusions:The method of determination of quinidine was reliable and suitable for blood samples determination in the clinical practice.