摘要
The key factors affecting pollen cryopreservation by vitrification in Brassica campestris var. purpurea were investigated. The factors involved the pollen maturity, the pollen resistance to dehydration, the components of vitrification solution(PVS), and the concentration of diluent. Thus, a suitable procedure was established for pollen vitrification, the maximum relative survival rates of mature (at the day of anthesis)and nearly mature(3 days before anthesis)pollen were about 80%, 63% respectively. This procedure has been also successfully applied to two other species ( Brassica napus, Brassica chinensis ). The advantage of cryopreservation of pollen by vitrification was discussed.
The key factors affecting pollen cryopreservation by vitrification in Brassica campestris var. purpurea were investigated. The factors involved the pollen maturity, the pollen resistance to dehydration, the components of vitrification solution(PVS), and the concentration of diluent. Thus, a suitable procedure was established for pollen vitrification, the maximum relative survival rates of mature (at the day of anthesis)and nearly mature(3 days before anthesis)pollen were about 80%, 63% respectively. This procedure has been also successfully applied to two other species ( Brassica napus, Brassica chinensis ). The advantage of cryopreservation of pollen by vitrification was discussed.
