产纤维素酶细菌的选育研究

STUDIES ON THE SCREENING OF CELLULASE PRODUCING BACTERIA

以产黄纤维单胞菌ＣＬ１和芽孢杆菌ｘｘ－０１为出发株，经诱变处理后，在含葡萄糖的产酶培养基平板上筛选到能形成较大透明圈的抗分解代谢物阻遏突变株．其中ＣＬ１的突变株ＣＭＣ酶活可提高２．１倍（６０．１Ｕ／ｍｌ），微晶纤维素酶可提高３～４倍（３９．５Ｕ／ｍｌ），芽孢杆菌ｘｘ－０１的ＣＭＣ酶活可提高５．０倍（７８０Ｕ／ｍｌ）。

Cellulomonas flavigena CL 1 and Bacillus xx-01 were chosen as original strains. The strains were continually mutagenized, catabolite repression-resistant mutants were selected on the producing enzyme medium plate in the presence of excessive glucose. These mutants can shape bigger transparent ring, Cx activity of CL 1 mutant can increase 2-1 times (60.1U/ml), avicelase activity can increase 3-4 times (39.5U/ml), Bacillus xx-01 C x activity can increase 5 times (780U/ml), and was studied situation of CL 1 constitutive mutant on hydrolyzed natural cellulose.