骨巨细胞瘤不同灭活方法的实验研究

Experimental Studiesof Giant - cell Tumor of Bone in Different Destroying Ways

采用6例Ⅰ、Ⅱ级骨巨细胞瘤病人节段截肢的组织,每例均制成16块1.5cm^3的肿瘤组织块,以16种不同灭活方法处理。灭活后在距离瘤组织表面2.5mm、5.0mm、7.5mm处取材,行光、电镜观察。观察结果表明:75％酒精,95％酒清,液氮冷冻,电凝烧灼仅对骨巨细胞组织块2.5mm以外有灭活作用。在碳酸、50％氯化锌、骨水泥灭活深度同达5.0mm。在此基础上,加用酒精或延长浸泡时间灭活程度不再加深。88℃水煮沸对骨巨细胞瘤的灭活最为彻底。50％氯化锌加石碳酸、石碳酸加骨水泥,50％氯化锌加骨水泥灭活效果比较理想,其灭活效果仅次于88℃水煮沸,适用于临床病灶清除后骨内壁的涂擦,是三种值得推广的灭活方法,但其机理有待于进一步探讨。

Limbs were taken from six patients with giantcell tumou of bone which is concernedwith I II grade. Each of tumor tissues were divided intosirteen blocks which is 1. 5cm3 in size, Eye-ryblocl swere handled respectively in sixteendifferent ways, Arter the tuner tissues weredesfroied, samples were remcved from a depth 2. 5rnm5.0mm and 7. 5mm and observed under lightmicroscopes and electron microscopes. The resultshowed that depth of the destroying by 75% percent. 95% percent ethyl alcohol. nirogen liquid andelectric scorch reach 2. 5mm . Depth of thedestroying by carbolic acid. Zinc chloride (50%) . reinforced concrete reach 5.0mm . After above - mentioned procedure , thd tumor tissues weresoaked again with ethyl alcohol or time of sockingwas prolonged , thd depth of destroying doesn'tdecelope. Water of 88C in destroying is thd best. Carbolic acid combined With Zinc Chloride ( 50%) , Carbolic acid combined with reinforced concrete. Zinc Chloride ( 50%) . combined With reinforcedconcrete have thd desired effect, but they ares-lightly less than water of 88C . They are adaptedto Smear in partital of bone after thd foci oftumor is cleaned away. The three ways are worth of usefulness widely. But thd mechanism requires tomake furtyer research in future.