乳腺癌患者3p24等位基因杂合缺失的研究

Study on Loss of Heterozygosity of Chromosome 3 (3p24) in Breast Cancer

应用聚合酶链反应（ＰＣＲ）技术，配合限制片段长度多态性（ＲＦＬＰ）分析，对２２例妇女乳腺癌患者３号染色体短臂３ｐ２４等位基因杂合缺失（ＬＯＨ）进行了测定。结果显示在３ｐ２４ＥＡβＭＤ位点和ＥＡβＨ位点的ＬＯＨ检出情况为６／１４，杂合缺失率为４２．８％。实验发现乳腺癌患者在３ｐ２４位点的ＬＯＨ较高，提示这一位点可能存在抑癌基因，此基因的缺失改变与乳腺癌的发生可能有密切关系。

Restriction fragment length polymorphisms analysis was performed with the polymerase chain reaction to study loss of heterozygosity (LOH) from chromosome 3(3p24) in 22 breast cancer patients. Deletion mapping in these tumors indicated that the LOH was 42.8%( 6/14) within band 3p24 EAβMD or EAβH locus. These results suggested that there were one or more putative tumor suppressor genes at this site. It may contribute to the pathogenesis of breast cancer.