摘要
The purpose of this study were to confirm whether the modified treatment with L-glutamic acid could attenuate the calcification of the GA-fixed valves and improve its biocompatibility. Pericardial valves were routinely treated with GA and valves were treated with GA and 8% L-glutamic acid. The valves treated with these methods were implanted subcutaneously in rats. Calcium deposits of the valves collected at the 7th, 21st, 60th, 90th day were assessed by atomic absorption spectroscopy, and the pathologic changes were examined by light and electron microscopy. Cultured endothelial cells (ECs ) were seeded onto the valves. The cell counts were determined at the 1st. 4th, 7th, 10th day after seeding. PGI2 in culture medium was tested at the 10th day. Transmission and scanning electron microscopy were used to observe the growth of ECs on the valves.Results showed that subsequent treatment with L-glutamic acid could significantly mitigate calcification of bovine pericardial valves as compared with simple GAfixed valves (P<0. 01). ECs seeded on the GA treated valves died within 4 days.On the valves treated by modified method, ECs could proliferate and release PGI2. It is concluded that treatment with L- glutamic acid can markedly inhibit the calcification and improve the biocompatibility of bioprosthetical valves.
The purpose of this study were to confirm whether the modified treatment with L-glutamic acid could attenuate the calcification of the GA-fixed valves and improve its biocompatibility. Pericardial valves were routinely treated with GA and valves were treated with GA and 8% L-glutamic acid. The valves treated with these methods were implanted subcutaneously in rats. Calcium deposits of the valves collected at the 7th, 21st, 60th, 90th day were assessed by atomic absorption spectroscopy, and the pathologic changes were examined by light and electron microscopy. Cultured endothelial cells (ECs ) were seeded onto the valves. The cell counts were determined at the 1st. 4th, 7th, 10th day after seeding. PGI2 in culture medium was tested at the 10th day. Transmission and scanning electron microscopy were used to observe the growth of ECs on the valves.Results showed that subsequent treatment with L-glutamic acid could significantly mitigate calcification of bovine pericardial valves as compared with simple GAfixed valves (P<0. 01). ECs seeded on the GA treated valves died within 4 days.On the valves treated by modified method, ECs could proliferate and release PGI2. It is concluded that treatment with L- glutamic acid can markedly inhibit the calcification and improve the biocompatibility of bioprosthetical valves.
