脂肪乳剂对人肠道相关淋巴组织的影响

Effects of lipid emulsion on gut-associated lymphoid tissue in human

探讨脂肪乳剂对胃肠道肿瘤患者肠道相关淋巴组织的影响。方法：20例胃肠道癌症患者随机分为含脂组（n＝10）和无脂组（n＝10），分别接受全肠外营养（TPN）支持7天后，分离肠系膜淋巴结和肠粘膜固有层的淋巴细胞，调细胞浓度至1×109／L，以植物血凝素（PHA）诱导培养48小时，测其上清液中肿瘤坏死因子α（TNF－α）和可溶性白介素-2受体（sIL-2R）含量。结果：含脂组肠系膜淋巴结淋巴细胞中TNF－α显著降低（P＜0．05），而肠粘膜固有层淋巴细胞中sIL-2R显著增高（P＜0．05）。结论：脂肪乳剂对肠道相关淋巴组织的诱导部位和效应部位均有抑制作用。

Objective: To study the effects of lipid emulsion on gut-associated lymphoid tissue in gastrointestinal carcinoma. Methods: 20 patients with gastrontestinal carcinoma were randomly divided into containing lipid total parenteral nutrition group (Lipid-TPN, n = 10) and no-lipid TPN group (n =10).After 7 days of TPN, mesenteric lymphoid nodes lymphocytes (MLNL) and lamina propria lymphocytes (LPL) were is olated respectively. Cell counts were adjusted to 1 x 109 cells/ml in RPMI 1 640 with 10% fatal calf serum. Then they were stimulated and cultured with phtohemagglutinin (PHA) for 48h.Concentrations of tumor necrosis factor-alpha (TNF-α) and soluble interleukin-2 receptors (sIL-2R) in the supernatants were measured. Results: There were significant decrease in TNF-α from MLNL and LPL in lipid-TPN group (P < 0. 05 ). sIL-2R levels of LPL increased significantly in lipid-TPN group (P < 0.05). Conclusion: Lipid emulsion could suppress both affector and effector limbs of the gut-associated lymphoid tissue.