利用微卫星标记研究新生隐球菌分子流行病学

Study on molecular epidemiology of Cryptococcus neoformans species using microsatellite marker

目的研究国内隐球菌临床分离株的遗传多态性和分子流行病学。方法选择与新生隐球菌遗传相关的9个微卫星标记,分析这9个位点从1993～2009年国内分离到的新生隐球菌临床株遗传背景、来源及变异程度。结果 116株被研究的隐球菌临床分离株,主要归属于3个微卫星复合物(MC2,MC3和MC12),其中大部分为MC2(103株)。8株菌株属于目前为止未被国内外认识的新复合物(MC12)。结论利用微卫星DNA多态性研究新生隐球菌分子流行病学有较大的应用价值。

Objective To study the molecular epidemiology of clinical isolates of Cryptococcus neoformans in China.Methods Totally 116 clinical strains of Cryptococcus neoformans from 1993 to 2009 were isolated and the epidemiology was analyzed by microsatellite genotyping（STR）.Results All the 116 strains of C.neoformans belonged to three microsatelite complexes（MCs）,with the majority of MC2（n=103）,and eight isolates belonged to an unknown microsatellite complex which was labeled as MC12.Conclusions STR is valuble in mocular epidemiological study on Cryptococcus neoformans.