摘要
采用聚丙烯酚胺凝胶垂直板电泳结合光密度扫描法，分析在四种镉中毒浓度（0．2、04、0．8和1．6mg／kg）下的SD大鼠睾丸中乳酸脱氨酶（LDH）同工酶活性的变化。结果表明：LDH各同工酶的活性在对照组和四种镉染毒组之间均有显著性差异（P＜0．05），LDH－X酶活性受锻中毒抑制最明显，LDH1、LDH2、LDH3和LDH4酶活性随镉中毒浓度的升高而逐渐抑制，而LDH5酶的活性在镉浓度0．2～0．8mg／kg范围内时升高而在1．6mg／kg剂量时则下降的特殊反应，LDH同工酶总活性随镉中毒浓度的升高而降低。结果提示：镉可能对LDH同工酶H、M和C亚基的基因表达影响不一。
This paper analysed the activities of LDH isozymes in the testes of SD rats exposed tofour different cadimium (Cd) concentrations (O. 2. 0. 4. 0. 8 and 1. 6mg/kg) with the methods of verticalstab of polyacrylamic gel electrophoretics and spectrophometrics. The results showed that there wassignificant difference (P<0. 05) in the activity of every LDH isozyme between the normal team and eachof four Cd poisoned teams. The activity of LDH -X was the most significantly inhibited among LDHisozymes by Cd. The activities of LDH,, LDH2, LDH,and LDH4 were gradually inhibited by Cd, whereas,that of LDH, increased from 0. 2 to 0. 8mg/kg Cd and decreased in 1. 6mg/kg Cd. The total activity ofLDH isozymes decreased in proportion to Cd effects on three-gene expressions of H, M and C subunits inLDH isozymes.
出处
《实用临床医药杂志》
CAS
1997年第1期15-17,共3页
Journal of Clinical Medicine in Practice
