摘要
lambda-DNA Hind III was denatured by HCl, renatured by NaOH, and digested by DNase I sequentially. The produced mixture was separated with Sephadex G200 column, and the chromatograph showed a very weak peak of one component resistant to the digestion of DNase I. The results of AFM studies on this component indicated that an unusual form of DNA was present, with the height 13 times that of double-stranded DNA. By comparison with known results, it is suggested that this is a multi-stranded DNA other than simple supercoils.
lambda-DNA Hind III was denatured by HCl, renatured by NaOH, and digested by DNase I sequentially. The produced mixture was separated with Sephadex G200 column, and the chromatograph showed a very weak peak of one component resistant to the digestion of DNase I. The results of AFM studies on this component indicated that an unusual form of DNA was present, with the height 13 times that of double-stranded DNA. By comparison with known results, it is suggested that this is a multi-stranded DNA other than simple supercoils.
