摘要
ＭｏｒｐｈｏｌｏｇｉｃａｌａｎａｌｙｓｉｓｏｆｌｙｍｐｈｖｅｓｓｅｌｓａｎｄｃａｐｉｌｌａｒｉｅｓｉｎｇａｓｔｒｉｃｃａｒｃｉｎｏｍａＬＩＡＯＸｉｎ＿Ｂｏ１，ＴＡＮＧＷｅｉ＿Ｐｉｎｇ１，ＺＨＡＮＧＱｉｎ＿Ｍｉｎｇ１，ＦＵＺｈｉ＿Ｇａｎｇ２ａｎｄＺＨ...
AIM To investigate the morphology of lymph vessels and capillaries in human gastric carcinoma and their peritumoral tissues and its relations with lymphous metastasis. METHODS The morphology and the fine distribution of lymph vessels and capillaries in and around the primary foci of gastric carcinoma were studied by 5′ Nase Alpase double staining method, the total amount, the opened amount and the opened rate of lymph vessels and capillaries were counted under light microscope (100×), and the maximal lumen area, perimeter and diameter were measured with image analysis technique. RESULTS Under light microscope, lymph vessels and capilla_ ries showed strong 5′ Nase positive reaction with brown and dark brown staining, while blood vessels and capillaries revealed significant Alpase activity with blue staining. There were a lot of lymph vessels, capillaries and solid strip like tissues in gastric carcinoma. The total amount of lymphatics in metastatic group (gastric carcinoma vs peritumoral tissue) and non_metastastic group was (26 9±14 2 vs 10 4±4 0, 11 4±3 4 and 9 7±3 2, P <0 01). Their opened rates were 21 2 vs 47 5, 40 4 vs 46 0 ( P <0 01). The maximal lumen area (1502 98±1236 91 vs 5526 80±4853 42; 1918 14±2299 24 vs 3836 16±3549 16; 5526 80±4853 42 vs 3836 16±3549 16, P <0 05), the perimeter (220 33±130 25 vs 441 43±276 51; 241 79±171 13 vs 333 80±199 66; 441 43±276 51 vs 333 80±199 60, P <0 05), and the diameter (28 80±14 98 vs 59 39±28 53; 25 37±15 79 vs 46 22±20 85; 59 39±28 53 vs 46 22±20 85, P <0 05) of lymphatics in gastric carcinoma in metastatic group were lower than those in other groups. CONCLUSION There are new born lymph capillaries in gastric carcinoma. The dilation of lymph capillaries may be related to edema in peritumoral connective tissues. The lymph node metastasis of gastric carcinoma occurs through invading mature lymph capillaries in and around the primary foci of gastric carcinoma.
