摘要
AIM To investigate the clinical significance of the PCR assay in the diagnosis of gastric H. pylori infection. METHODS HP infection in gastric antral biopsied specimens was identified by the polymerase chain reaction (PCR) to amplify the specific HP urease gene fragments (PCR HP DNA) in 154 patients with gastrointestinal disorders. HP urease genes oligonucleotide primers specific for HP (16s rRNA) were used. Urease test and ELISA for serum anti HP IgG were also used as control. RESULTS PCR HP DNA was detected in 140(91%) of the 154 patients, 114 and 125 were found infected with HP by urease test and ELISA HP IgG, respectively. There was a marked difference in the HP positive rate between the PCR HP DNA and the urease test or ELISA HP IgG ( P <0 05). The rate of HP infection increased with age although a minority of infected people develop signs and symptoms of gastric disorders. HP infection is closely related to adenocarcinoma in gastric antrum and the downbody of the stomach. CLNCLUSION PCR is a sensitive and specific method for the detection of HP in human gastric tissues. Detection of HP DNA in vivo by this approach might improve the clinical diagnosis and epidemiological research of H. pylori infection.
