摘要
ＲｅｌａｔｉｏｎｓｈｉｐｂｅｔｗｅｅｎｌｏｓｓｏｆｈｅｔｅｒｏｚｙｇｏｓｉｔｙｏｆｍｉｃｒｏｓａｔｅｌｉｔｅｏｎＤＣＣｇｅｎｅａｎｄｐｒｏｇｎｏｓｉｓｏｆｃｏｌｏｒｅｃｔａｌａｄｅｎｏｃａｒｃｉｎｏｍａＺＨＡＯＰｏ１，ＨＵＹｉｎｇＣｈｕａｎ２...
AIM To investigate the relationship between the loss of heterozygosity (LOH) of microsatellite on the Deleted in colorectal carcinoma (DCC) gene and the prognosis of colorectal adenocarcinoma. METHODS A retrospective study of 58 cases of colorectal adenocarcinoma with the follow up data and paired control normal mucosal tissues from 1983 to 1985 from files of the Department of Pathology, West China University of Medical Sciences was carried out by PCR microsatellite method. Tumors were graded as well, moderately and poorly differentiated in 16, 35 and 7 cases, and staged as Dukes′ A, B, C in 11, 30 and 17 cases, respectively. RESULTS Heterozygosity of microsatellite was lost on DCC gene in 18 of 58 (31 0%) cases of colorectal adenocarcinoma. The 5 year survival rate between the positive and negative for LOH was 44 4% and 77 5%, respectively, being significantly different ( P <0 05). It was suggested that the LOH on microsatellite of DCC gene was correlated to the prognosis, but not to the differentiation ( P >0 05) and Dukes′ stage ( P >0 05) in colorectal adenocarcinoma. CONCLUSION LOH of microsatellite on DCC gene may be one of malignant markers. Combined with the traditional prognostic indices, LOH can predict the prognosis of colorectal adenocarcinoma.
