AgNOR and rasp21 expression in gastric mucosal lesions with Hellicobacter pylori infection

AgNOR and rasp21 expression in gastric mucosal lesions with Helicobacter pylori infection

ＡｇＮＯＲａｎｄｒａｓｐ２１ｅｘｐｒｅｓｓｉｏｎｉｎｇａｓｔｒｉｃｍｕｃｏｓａｌｌｅｓｉｏｎｓｗｉｔｈＨｅｌｉｃｏｂａｃｔｅｒｐｙｌｏｒｉｉｎｆｅｃｔｉｏｎＧＡＯＨｅｎｇＪｕｎ，ＬＵＸｉｕＺｈｅｎ，ＺＨＡＮＧＸｉａｏＹｏｎｇａｎｄＺＨＡＯＺｈｉ...

IM To study the number of AgNOR and rasp21 expression in different gastric mucosal lesions with Helicobacter pylori (Hp) infection to evaluate their biological behaviour and possible mechanism of Hp. METHODS Hp (using CLO test combined with WathinStarry staining), AgNOR (silver colloid technique) and rasp21 (monoclonal antibody and immunohistochemical staining—ABC method) were detected in 278 patients with endoscopically and pathologically confirmed gastric mucosal lesions, including chronic superficial gastritis (CSG), chronic atrophic gastritis (CAG), intestinal metaplasia (IM), dysplasia (Dys), and gastric cancer (GC). Among them, 146 cases were Hp positive, and 132 cases Hp negative. RESULTS The mean number of AgNOR in Hp positive group was significantly higher than that in Hp negative group in the gastric mucosal lesions except for CSG (P<005 or P<001). The positive rate of rasp21 expression in Hp positive group was also significantly higher than that in Hp negative group in gastric mucosal lesions except for CSG and CAG (P<005).CONCLUSION Hp+ gastric mucosal lesions have more biological behaviour of tumors. Hp may act as a promoter to activate ras gene and to stimulate cell over proliferation.