摘要
IM To investigate the mechanisms of antiliver fibrosis actions of Fuzheng Huayu (FZHY, strengthen the body resistance and promote blood circulation) decoction.METHODS Ito cells were isolated from rats and cultured, the sera were collected from the normal rats after administration of FZHY decoction and added to the subcultured cells. Ito cell morphology was observed under the contrast microscopy, cells viability was measured by Pro incorporation, proliferation by TdR incorporation and MTT colorimetric assay, collagen synthesis rate by Pro incorporation and collagenase digestion. The effects of FZHY decoction on Ito cell morphology and functions were observed.RESULTS The rat serum containing FZHY decoction had no influence on Ito cell morphology, but could improve the cell viability. It could markedly inhibit Ito cell proliferation and collagen synthesis, these effects tend to be drug dose and drug sera concentration dependent.CONCLUSION Seropharmalogical method can be efficiently used to investigate the pharmacological mechanism of antifibrotic traditional Chinese herbs and formulae. The inhibition of Ito cell proliferation and collagen synthesis may be one of the major mechanisms of the antifibrosis actions in FZHY decoction.
