摘要
Chemical synthesis and cloning of human calcitonin (hCT) cDNA. Methods and Results:The cDNA sequence coding for human calcitonin (hCT) was divided into 6 fragments, synthesized by ABI 391 DNA synthesis instrument and cloned into PGEM7Z(+) vector following phosphorylation, annealing and ligation of the 6 fragments. The sequence of cloned hCT cDNA was proved to be the same as designed by double DNA sequencing. Conclusion: A reliable and forthright method of chemical synthesis and cloning of oligo-peplides cDNA (or gene) was provided in this study. This paper is a basic work for expression of hCT cDNA.
Chemical synthesis and cloning of human calcitonin (hCT) cDNA. Methods and Results:The cDNA sequence coding for human calcitonin (hCT) was divided into 6 fragments, synthesized by ABI 391 DNA synthesis instrument and cloned into PGEM7Z(+) vector following phosphorylation, annealing and ligation of the 6 fragments. The sequence of cloned hCT cDNA was proved to be the same as designed by double DNA sequencing. Conclusion: A reliable and forthright method of chemical synthesis and cloning of oligo-peplides cDNA (or gene) was provided in this study. This paper is a basic work for expression of hCT cDNA.
